This study sought to assess the challenges and opportunities encountered by women entrepreneurs in Papua New Guinea. The study covered two provinces, namely the Central and Morobe, and included 165 registered and unregistered women entrepreneurs. The sample was obtained using simple random sampling. The chi-square test and correlation analysis were employed for data analysis. The study revealed that female entrepreneurs in the two provinces encountered challenges including limited access to education, insufficient awareness of training opportunities, inadequate financial assistance, gender disparities, and instances of violence against women. These challenges were found to be undermining women entrepreneurs' enthusiasm to participate in the mainstream economy. Additionally, it was observed that several institutions and non-governmental organizations (NGOs) were taking the initiative to offer entrepreneurial education and increase awareness, specifically targeting communities residing in distant places that lack access to basic internet and other facilities. Due to these obstacles, women in Papua New Guinea persistently encounter various challenges. The study aimed to identify and analyze the key challenges and opportunities women entrepreneurs face in Papua New Guinea and propose effective strategies for their empowerment and economic advancement